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Protein Expression with Western Blotting

Western Blotting Workflow

Instruction for all steps in the western blotting workflow.
Introduction:
The western blot (also called the protein immuno-blot), or western blotting, is a widely used analytical technique in molecular biology, and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. It is a powerful technique that enables the detection of the proteins, estimation of the quantities and determination of their molecular weights.

The entire western blotting workflow is complicated, which includes several individual steps, each of which is important for you to get the high-quality data.
SHST provides the related devices for the critical steps, let's take an overview on this page.
 
SHST's Solution:

Sample preparation (Grinder-->High Speed Centrifuge--->Samples Extraction)

Electrophoresis (Vertical Electrophoresis)

Blotting (Wet/Tank blotting Systems, Bench Shakers for Membranes washing & Western blot blocking)

Detection and image acquisition (ECL or Dyes, Western blot Imaging System for Chemiluminescence and Fluorescence Detection)

Image Analysis and Quantification

Sample Preparation

Electrophoresis

Transfer

Detection

Image Acquisition

Image Analysis and Quantification

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